Production Optimization and Industrial Applications of Amylase From Indigenous Bacterial Species Using Banana Peels
Keywords:Alpha-amylase;, Bacillus megaterium, Exiguobacterium auranticum, submerged state fermentation, banana peels
Introduction: Alpha amylases are starch hydrolyzing enzymes that possess high industrial demand. Various strategies have been adopted to enhance cost-effective enzyme production of which utilization of agro-industrial waste is very promising. Methodology: In this study two amylase producers Bacillus megaterium and Exiguobacterium auranticum were isolated and identified by 16sRNA sequencing their growth conditions were optimized via submerged fermentation using banana peels as carbon source. Bacillus megaterium found to be mesophilic alkaline (37℃, pH of 9) strain giving 472 U/ml while Exiguobacterium auranticum found to be acidic thermophilic (50°C, pH 5) that gave 391 U/ml. Results and Conclusion: Production of amylase by acidic thermophilic E. auranticum is reported here for the first time. The crude enzyme also showed better chocolate and curry stain removal capacity when combined with commercial detergent. Also juice clarification assay showed promising results which indicates that the enzymes could be potentially used in detergent and food industry.
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