Production Optimization and Industrial Applications of Amylase From Indigenous Bacterial Species Using Banana Peels
DOI:
https://doi.org/10.46568/bios.v4i1.75Keywords:
Alpha-amylase;, Bacillus megaterium, Exiguobacterium auranticum, submerged state fermentation, banana peelsAbstract
Introduction: Alpha amylases are starch hydrolyzing enzymes that possess high industrial demand. Various strategies have been adopted to enhance cost-effective enzyme production of which utilization of agro-industrial waste is very promising. Methodology: In this study two amylase producers Bacillus megaterium and Exiguobacterium auranticum were isolated and identified by 16sRNA sequencing their growth conditions were optimized via submerged fermentation using banana peels as carbon source. Bacillus megaterium found to be mesophilic alkaline (37℃, pH of 9) strain giving 472 U/ml while Exiguobacterium auranticum found to be acidic thermophilic (50°C, pH 5) that gave 391 U/ml. Results and Conclusion: Production of amylase by acidic thermophilic E. auranticum is reported here for the first time. The crude enzyme also showed better chocolate and curry stain removal capacity when combined with commercial detergent. Also juice clarification assay showed promising results which indicates that the enzymes could be potentially used in detergent and food industry.
References
Singh R, Kumar M, Mittal A, Mehta PK. Microbial enzymes: Industrial progress in 21st century. 3 Biotech 2016; 6(2): 1-15.
Deljou A, Arezi I, Khanahmadi M. Scale-up thermostable α-amylase production in lab-scale fermenter using rice husk as an elicitor by Bacillus licheniformis-AZ2 isolated from Qinarje Hot Spring (Ardebil Prov. of Iran). Periodicum Biologorum 2018; 120(1): 11-21.
Saini R, Saini HS, Dahiya A. Amylases: characteristics and industrial applications. J Pharmacog Phytochem 2017; 6(4): 1865-71.
Tallapragada P, Dikshit R, Jadhav A, Sarah U. Partial purification and characterization of amylase enzyme under solid state fermentation from Monascus sanguineus. J Genetic Eng Biotechnol 2017; 15(1): 95-101.
Anbu P, Gopinath SC, Chaulagain BP, Lakshmipriya T. Microbial enzymes and their applications in industries and medicine 2016. Biomed Research International. 2017;2017:2195808.
Far BE, Ahmadi Y, Khosroshahi AY, Dilmaghani A. Microbial alpha-amylase production: Progress, challenges and perspectives. Adv Pharm Bullet 2020; 10(3): 350-58.
Bharathiraja S, Suriya J, Krishnan M, Manivasagan P, Kim SK. Production of enzymes from agricultural wastes and their potential industrial applications. In: Advances in Food and Nutrition Research 2017; 80: 125-48. Academic Press.
Pandey A, Soccol CR. Economic utilization of crop residues for value addition: a futuristic approach. J Sci Indus Res 2000; 59(1): 12-22.
Sagar NA, Pareek S, Sharma S, Yahia EM, Lobo MG. Fruit and vegetable waste: Bioactive compounds, their extraction, and possible utilization. Comp Rev Food Sci Food Safety 2018; 17(3): 512-31.
Aslam M. Agricultural productivity current scenario, constraints and future prospects in Pakistan. Sarhad J Agric 2016; 32(4): 289-303.
Noor MI, Sanaullah N, Ali LB. Economic efficiency of banana production under contract farming in Sindh Pakistan. J Global Eco 2015; 3(166): 1-5.
Guerrero R. Bergey's manuals and the classification of prokaryotes. Int Microbiol 2001; 4(2): 103-9.
Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: Molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 2018; 35(6): 1547-9.
Sondhi S, Kaur PS, Sura H, Juglani M, Sharma D. Amylase based clarification of apple, orange and grape juice. Int J Contemporary Technol Res 2021; 3(2): 187-90.
Sachdev S, Ojha SK, Mishra S. Bacillus spp. amylase: Production, isolation, characterization and its application. Int J Appl Sci Biotechnol 2016; 4(1): 3-14.
Salman T, Kamal M, Ahmed M, Siddiqa SM, Khan RA, Hassan A. Medium optimization for the production of amylase by Bacillus subtilis RM16 in shake-flask fermentation. Pak J Pharm Sci 2016; 29(2): 439-44.
Sivaramakrishnan S, Gangadharan D, Nampoothiri KM, Soccol CR, Pandey A. a-Amylases from microbial sources–an overview on recent developments. Food Technol Biotechnol 2006; 44(2): 173-84.
Jana M, Maity C, Samanta S, Pati BR, Islam SS, Mohapatra PK, Mondal KC. Salt-independent thermophilic α-amylase from Bacillus megaterium VUMB109: An efficacy testing for preparation of maltooligosaccharides. Indus Crops Products 2013; 41: 386-91.
Hagihara H, Igarashi K, Hayashi Y, Endo K, Ikawa-Kitayama K, Ozaki K, et al. Novel α-amylase that is highly resistant to chelating reagents and chemical oxidants from the alkaliphilic Bacillus isolate KSM-K38. Appl Environ Microbiol 2001; 67(4): 1744-50.
Vijayaraghavan P, Kalaiyarasi M, Vincent SG. Cow dung is an ideal fermentation medium for amylase production in solid-state fermentation by Bacillus cereus. J Genet Eng Biotechnol 2015; 13(2): 111-7.
Mojallali L, Shahbani Zahiri H, Rajaei S, Akbari Noghabi K, Haghbeen K. A novel∼ 34‐kDa α‐amylase from psychrotroph Exiguobacterium sp. SH 3: production, purification, and characterization. Biotechnol Appl Biochem 2014; 61(2): 118-25.
Sen SK, Jana A, Bandyopadhyay P, Mohapatra PK, Raut S. Thermostable amylase production from hot spring isolate Exiguobacterium sp: a promising agent for natural detergents. Sustainable Chem Pharm 2016; 3: 59-68.
Sundarram A, Murthy TP. α-amylase production and applications: a review. J Appl Environ Microbiol 2014; 2(4): 166-75.
Bidlas E, Du T, Lambert RJ. An explanation for the effect of inoculum size on MIC and the growth/no growth interface. Int J Food Microbiol 2008; 126(1-2): 140-52.
Chapman J, Ismail AE, Dinu CZ. Industrial applications of enzymes: Recent advances, techniques, and outlooks. Catalysts 2018; 8(6): 238.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 BioSight

This work is licensed under a Creative Commons Attribution 4.0 International License.