Prevalence Of Blood Groups And Association With Ailments In Pakistani Population: A Review of The Current Literature


  • Saadia Saad Department of Physiology, Baqai University, Karachi, Pakistan
  • Syed Tousif Ahmed Department of Physiology, Ziauddin University, Karachi, Pakistan
  • Ambrina Khatoon Department of Research, Ziauddin University, Karachi, Pakistan
  • Jawaid Ansari Department of Cardiology, Ziauddin Hospital, Karachi, Pakistan
  • Asaad J Mirza Department of Operative Dentistry, Baqai Medical University, Karachi, Pakistan



Antigen, ABO, blood donors, blood groups, Rh factor, phenotype


The aim of this review is to report studies performed in Pakistan on the prevalence of blood groups among various sections of the population and their association with human diseases.  Google Scholar was searched for the last 20 years based on MESH keywords mentioned below. The inclusion criteria were the studies published in the new millennium that reported either prevalence of blood groups or their association with diseases in the Pakistani population.

There were 50 studies in Pakistan which fulfill the inclusion criteria. Among them, twenty- six were from Punjab, seven were from Khyber Pakhtunkhwa (KpK), six from Sindh, four from Kashmir, two from Northern areas, one from Baluchistan and 4 are review articles. This review concludes that blood group B is prevalent among Pakistani population followed by O, A and AB. Studies done in Azad Jammu Kashmir (AJK) and Gilgit- Baltistan reveal that B and A is prevalent in these areas respectively. AB is the least found group and Rh+ve is common among all studies. Moreover, this review also shows that blood groups act as a risk factor for many diseases. Therefore, Pakistani researchers are suggested to work on the genetic makeup of these blood groups to find out the cause of association with systemic ailments in the country.


Pramanik T, Pramanik S. Distribution of ABO and Rh blood groups in Nepalese medical students: A report. East Mediterr Health J 2000; 6: 156-8

Sidhu S. Distribution of the ABO blood groups and Rh (D) factor among the scheduled caste population of Punjab group markers among the four scheduled caste. Anthropologist 2003; 5: 203-4. https: //

Khichi QK, Ali SA, Malik MS. Prevalence of ABO and Rh (D) blood groups in Bahawalpur division. Pak Ped J 2000; 24(1): 1-2. https: //

Hussain A, Sheikh SA, Haider M, Rashied T, Malik MR. Frequency distribution of ABO and rhesus blood groups in population of Baluchistan, Pak Armed Forces Med J 2001; 51 (1): 22-6

Hameed A, Hussain W, Ahmed J, Rabbi F, Qureshi JA. Prevalence of Phenotypes and Genes of ABO and Rhesus (Rh) Blood Groups in Faisalabad, Pakistan. Pak J Biol Sci 2002; 5: 722-4

Ghori MU, Tayyab M, Raziq F. Frequency of ABO and RhD Blood Groups in Transfusion Dependent Patients. J Postgrad Med Inst 2003; 17(2): 177-83. https: //

Khan MS, Tahir F, Sheikh MA, Mubashir Ahmed Sheikh, Subhan F, Kazi BM, Dil AS, Deepa F, Irshad Ali I, Baloch MK. An analysis of genotype frequencies of blood group antigens from Bannu region (NWFP) in Pakistan. Gomal J Med Sci 2004; 2(1): 1-5

Rahman M, Lodhi Y. Frequency of abo and rhesus blood groups in blood donors in Punjab, Pak J Med Sci 2004; 20(4): 315-8

Bhatti R. Distribution of ABO and Rhesus blood alleles in a strict endogamous subtribe of lower Sindh (Pakistan): Setharani (Tribe: Noohani). Pak J Zool 2004; 36(2): 125-8

Khan MS, Sibtain F, Tahir F, Kazi BM, Dil AS, Sultan S, Deepa F, Khan F. Prevalence of Blood Groups & Rh factor in Bannu region NWFP (Pakistan) Pak J Med Res 2004; 43(1): 8-10

Anees M, Mirza MS. Distribution of ABO and Rh blood group alleles in Gujrat region of Punjab, Pakistan. Proc Pakistan Acad Sci 2005; 42(4): 233-8.

Alam M. ABO and Rhesus blood groups in potential blood donors at Skardu (Northern Areas). Pak J Pathol 2005; 16: 94-97

Ali N, Anwar M, Bhatti FA, Nadeem M, Nadeem A, Ali AM. Frequency of ABO and RH blood groups in major ethnic groups and casts of Pakistan, Pak. J. Med. Sci 2005; 21 (1): 26-29

Rehman A, Khan MA, Ashraf M, Malik SA, Saeed MA, Rafique A, Ali A. ABO & Rhesus Blood Groups Professional Med J 2005; 12(4): 368-371

Khan MS, Farooq N, Qamar N, Tahir F, Subhan F, Kazi BM, et al. Trend of blood groups and Rh factor in the twin cities of Rawalpindi and Islamabad. J Pak Med Assoc 2006; 56(7): 299-302

Khalid M, Qureshi MA. Frequencies of blood group antigens and corresponding alleles in the population of Mirpur, Azad Jammu Kashmir, Pakistan. J Anim Pl Sci 2006; 16(3-4): 96-8

Anees M, Jawad A, Hashmi I. Distribution of ABO and Rh blood group alleles in Mandi Bahauddin district of Punjab, Pakistan. Proc. Pakistan Acad. Sci 2007; 44(4): 289-94

Khattak ID, Khan TM, Khan P, Shah SMA, Khattak ST, Ali A. Frequency of ABO and Rhesus blood groups in district Swat, Pakistan. J Ayub Med Coll Abbottabad 2008; 20(4): 127-9

Khan MN, Khaliq I, Bakhsh A, Akhtar MS, Din AM. Distribution of ABO and Rh D blood groups in the population of Poonch district, Azad Jammu and Kashmir. Eastern Mediterranean Health J 2009; 15: 3‎, 17-721

Iqbal M, Niazi A, Tahir M. Frequency of ABO and Rh blood groups in healthy donors. J Rawalpindi Med College 2009; 13(2): 92-4

Pasha AK, Hashir MM, Khawar S. Frequency of ABO blood groups among medical students. J Sur Pak 2009; 14(2): 93-5

Anees M, Jawad A. Distribution of ABO and Rh Blood Group Alleles in Sahiwal district of the Punjab, Pakistan. Proceedings of Pak Acad Sci 2011; 48 (1): 39-43

Chisti HM, Waheed U, Ansari MA, Wazir I, Hussain Z. ABO and Rh blood group phenotypes in Mirpur, Azad Kashmir, Pakistan. J Pub Health Biological Sci 2012; 1(2): 41-3

Shakir M, Khan SA, Ghani E. Frequency of ABO and Rh (D) blood groups among blood donors in Rawalpindi/Islamabad area. Pak. Armed Forces Med. J 2012; 62(2): 304-6

Anwar B, Kaleem F, Moazzam A, Rizvi SR, Karamat KA. Distribution of Blood Groups in Population of Lehtrar Road Islamabad. J Islamabad Med & Dent College 2013; 2(1): 13-6

Shams S, Afridi SG, Waddod A, Iqbal M, Munir M, Shams U, Khan M, Yousaf S. Frequency Distribution of Blood Groups ABO and RhD among the Students of Abdul Wali Khan University Mardan, Pakistan. Int J Agro Veterinary and Med Sci 2013;

Ilyas M, Iftikhar M, RasheedU. Frequency of ABO and Rh Blood groups in Gujranwala (Punjab), Pakistan. Biologia (Pakistan) 2013; 59(1): 107-14

Malik S, Din AM. Genetic Heterogeneity and Gene Diversity at ABO and Rh Loci in the Human Population of Southern Punjab, Pakistan. Pak J Zool 2013; 45(5): 1185-90

Khan MU, Bashir MW, Rehman R, Kian RA. Frequency of ABO and Rh (D) Blood Groups Among Blood Donors In Lahore, Pakistan. Int J Advanced Biological and Biomed Res 2014; 2(3): 2014: 597-600

Ullah S, Ahmad T. Distribution of ABO and Rh (D) Blood Groups in the Population of District Dir Lower, Khyber Pakhtunkhwa Pakistan. World Applied Sci J 2015; J33 (1): 123-135

Nazli R, Haider J, Khan MA, Akhtar T, Aslam H. Frequency of ABO blood groups and RhD factor in the female population of District Peshawar. Pak J Med Sci 2015; 31(4): 984-6

Ilyas M. Distribution of blood groups ABO and Rh reported from district Sialkot (Punjab) Pakistan. Sci Int 2015; 27(3): 2021-3

Rehman FU, Siddiqui MM, Nazir I, Khan MM, Zafar S, Ch ZA, Qadir I. Study of ABO and Rh-D Blood Groups among the Common people of Multan City Corporation area of Pakistan. Pak J Med Health Sci 2015; 9(3): 892-6.

Karim F, Moiz B, Muhammad FJ, Ausat F, Khurshid M. Rhesus and Kell Phenotyping of Voluntary Blood Donors: Foundation of a Donor Data Bank. J College of Physicians and Surgeons Pak2015; 25(10): 757-60

Ahmad S. Prevalence frequency of ABO and Rhesus blood groups in human in district Rahim Yar Khan, Pakistan. Am J Biosci 2015; 3(4): 141-4

Kanwal S, Qureshi HJ, Aslam MS, Masood S. Frequency of ABO and Rh blood groups in students of Akhtar Saeed Medical and Dental college, Lahore. Pak J Physiol 2016; 12(1): 29-30

Butt DS, Malik S, Khalid MZ, Aziz M, Humayun A. Gender Distribution of ABO and Rhesus Blood Groups among Medical Students of a Public Medical School in Lahore, Pakistan. Proceeding S.Z.P.G.M.I.2016; 30(2): 77-81

Saeed M, Hussain S, Arif M. Distribution of blood type and Rh factor among blood donors of Lahore. Pak Armed Forces Med J 2016; 66 (Suppl-3): S228-S232

Shaikh S, Shariq A, Zuberi AM. ABO and Rhesus blood group distribution in residents of Karachi. Pak J Med and Dent 2018; 7 (03): 32-5

Hussain I, Naqvi AN, Amir S, Abbas G, Mehdi Q. Blood groups distribution pattern among limited adult human population of district Gilgit, Gilgit-Baltistan, Pakistan. Int J Pure and Applied Zoology 2018; 6(2): 18-24

Butt SJ, Malik S, Malik H, Butt AJ. ABO and Rhesus Blood Grouping in Voluntary Blood Donors and its gender association. P J M H S 2018; 12(1): 268-71

Jabin F, Waheed U, Ahmed S, Arshad M, Arshad A, Zaheer HA. Red blood cell phenotyping of blood donors in Islamabad, Pakistan. Global J Transfusion Med 2018; 3(1): 26-9

Abbasi SQ, Sattar R, Mansoor G. Association of gender with ABO and Rh blood groups - a cross-sectional study. Pak J Physiology 2018; 14(3): 25-7

Khan MU, Shahzada N, Rehman R, Baig AA, Khan SA, Kiani RA. An overview of ABO and Rh blood group dissemination in general population of Azad Kashmir, Pakistan. Int J BioSci 2019; 15(5): 511-7

Ahmed M, Memon A, Iqbal K. Distribution pattern of ABO and Rhesus blood groups among different ethnic population of Karachi. J Pak Med Assoc 2019; 69(10): 1474-8

Noshkey A, Yazdani MS, Rathore MA, Hashmi IQJ, Hashmi AR, Hashmi KTK. Frequency Distribution of ABO and RhD Blood Groups amongst Blood Donors: A single centre study. P J M H S 2019; 13(3): 697-9

Irshad Z, Parween S, Kumar S, Anwar M, Shabbir A, Sultan BA. The ABO and Rhesus (RH) Blood Groups Distribution among the Female Blood Donors in Tertiary Care Hospital, Karachi. J Liaquat Uni Med Health Sci 2020; 19(01): 28-32

Nuzhat A. ABO blood groups are efficient in delineating relationships across Linguistic Subpopulations of Pakistan. Pak J Med Dentistry 2020, VOL. 9 (01)

Rehman. ABO and Rh (D) blood groups distribution in Pakistan: a systematic review. Forensic Res Criminol Int J 2020; 8(6): 237-44

Sabir, A., Iftikhar, A., Ijaz, M.U. et al. Retrospective study of frequency of ABO and Rhesus blood group among population of Safdarabad and Faisalabad cities of Pakistan. BMC Res Notes 2021; 14, 12. https: //

Rehman GU, Shi H. ABO and RH (D) Blood Groups Distribution In Pakistan: A Systematic Review. Int. J. Pure Appl. Zool 2021; 9(1): 1-9

Muhammad I, Saba N, Waheed U, Ullah W, Qasim M, Ahmed S, et al. A Systematic Review and Meta-Analysis on Prevalence of ABO and Rhesus Blood Groups in Pakistani Population 2022; 1: 110

Garratty G. Blood groups and disease: A historical perspective. Transfusion Med Rev 2000; 14(4): 291-301

Akhund IA, Alvi IA, Ansari AK, Mughal MA, Akhund AA. A study of relationship of ABO blood groups with myocardial infarction and angina pectoris. J Ayub Med Coll Abbottabad 2001; 13(4): 25-6

Wazirali H, Ashfaque RA, Herzig JW. Association of blood group A with increased risk of coronary heart disease in the Pakistani population. Pak J Physiol 2005; 1: 1-2

Lutfullah, Bhatti TA, Hanif A, Shaikh SH, Khan BZ, Bukhshi IA. ABO Blood Group Distribution and Ischemic Heart Disease. Annals 2011; 17(1): 36-40

Sharif S, Anwar N, Farasat T, Naz S. ABO blood group frequency in Ischemic heart disease patients in Pakistani population. Pak J Med Sci 2014; 30(3): 593-5

Siddiqui ZH, Chaudhry MA, Nigar M, Butt H. A study of association between ABO and Rh blood groups, sex, age and angina pectoris”, Sci. Int. (Lahore), 2011; 23(2): 113-6

Ashraf MZ, Khan IA, Zeeshan N, Chaudhry MA. Association of blood group A with ischemic heart disease. Biomedica 2017; 33(2): 131-5

Iftikhar I, Khan HS, Rehman AU. Acute Myocardial Infarction and its association with ABO blood groups in the Pakistani population. J Rawalpindi Medical College (JRMC); 2020; 24(2): 103-7

Noor A, Tashfeen S, Akhtar F, Anwar N, Din HU, Akhtar F. Association of COVID-19 with ABO blood groups in tertiary care center of Pakistan. Pak Armed Forces Med J 2020; 70 (COVID-19) (1): S251-55

Rahim F, Amin S, Bahadur S, Noor M, Mahmood A, Gul H. ABO / Rh-D Blood types and susceptibility to Corona Virus Disease-19 in Peshawar, Pakistan. Pak J Med Sci 2021; 37(1): 4-8.

Bari, A., Ch, A., Hareem, S., Bano, I., Rashid, J., & Sadiq, M. Association of Blood Groups with the Severity and Outcome of COVID-19 Infection in Children. J College Physicians Surgeons-Pakistan: JCPSP, 2021; 30(1): S57-S59

Rehman FU, Omair SF, Memon F, Rind BJ, Memon DA, Ali SA, et al. The relation of ABO blood group to the severity of coronavirus disease: A cross-sectional study from a tertiary care hospital in Karachi. Cureus, 2021, 13(7).

Burhan H, Hasan AS, Haque SM, Zaidi G, Shaikh T, Zia A. Association between blood group and susceptibility to malaria and its effects on platelets, TLC, and Hb. J Infect Dev Ctries 2016; 10(10): 1124-8

Shakeel HA, Maqsood H, Ali B, Khan AR. Association of chronic viral hepatitis with ABO blood groups and rhesus (Rh) factor. Int J Res Med Sci 2018; 6(4): 1114-8

Anwar M, Siddiqi GM, Haq S, Khokhar N, Jaffery G, Sharif N. Association of blood group types to hepatitis B and hepatitis C virus infection 2011.

S ALAM, Association of ABO, Rh blood group systems with lipids and other anthropometric Co variables as Predictors of Cardiovascular Risk in NWFP, Pakistan. ANNALS, 2004; 10(2).

Waseem AG, Iqbal M, Khan OA, Tahir M. Association of diabetes mellitus with ABO and Rh blood groups. Ann Pak Inst Med Sci 2012; 8(2): 134-136

Zaidi SSH, Ch. Ather AA, Kiran R, Syed U, Ashfaq U, Musharraf MU. Frequency of ABO blood groups among the Type II diabetes mellitus patients. Endocrinol Diabetes Res 2018; 4(2): 212-4

M. GhafarS. KhwajaM. ZahidS. I. HussainA. KarimA. Akram. Association of blood groups/Rh and diabetes mellitus in Karachi city, Pakistan. Brazilian J Biol 2024; 84: e252952.

Zahra M, Hussain A. Association between blood groups and refractive errors. Ophthalmology; 6(2): 26-28

Khan MI, Micheal S, Akhtar F, Naveed A, Ahmed A, Qamar R. Association of ABO blood groups with glaucoma in the Pakistani population. Canadian J Ophthalmol 2009; 44(5): 582-6.

Qadir MI and Hanif AZM. Blood Group System Connection with Depression. J Ment Disord Treat 2018; 4: 170. Doi: 10.4172/2471-271X.1000170

Iqbal S, Akram R, Akram S, Saif ullah M, Fatima QA, Afzal HMI. Association between ABO blood group phenotype and reaction to academic stress in young medical students. Int J Contempor Med Res 2019; 6(11): K6-K9

Abid K. Prevalence of ABO blood groups and their association with dust, pollen and skin allergy in young adults. Ann Pak Inst Med Sci 2015; 11(1): 12-5

Hashme RI, Khan NA. A study of fingerprints pattern in relation to ABO & Rh blood groups among medical students of AJK Medical College, Muzaffarabad. Med Forum 2014; 25(12): 7-10

Parveen N, Rehman J, Hassan SH, Hassan Z, Rehman M. Different blood groups; Association with body mass index in medical students of Karachi. Professional Med J 2016; 23(8): 1001-4.

Jawed S, Zia S, Tariq S. Frequency of different blood groups and its association with BMI and blood pressure among the female students of Faisalabad. Pak J Med Assoc 2017; 67(8): 1132-7.




How to Cite

Saadia Saad, Syed Tousif Ahmed, Ambrina Khatoon, Jawaid Ansari, & Asaad J Mirza. (2023). Prevalence Of Blood Groups And Association With Ailments In Pakistani Population: A Review of The Current Literature. BioSight, 4(1), 87–98.