Inhibitory potential of Phaseolus lunatus L. Seeds to Digestive Enzymes


  • Mehreen Zaheer Department of Pharmacognosy, Jinnah University for Women, Nazimabad, Karachi-74600, Pakistan
  • Anum Kalim Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, University of Karachi
  • Shah Ali Ul Qadar Department of Biochemistry, University of Karachi
  • Salman Ahmed Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, University of Karachi, Karachi, Pakistan
  • Muhammad Mohtasheem Ul Hasan Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, University of Karachi, Karachi, Pakistan



Phaseolus lunatus, α-amylase, maltase, glucoamylase, diabetes


Introduction: Study was taken with an objective to investigate the inhibitory effect of Phaseolus lunatus seeds extract on three enzymes (α-amylase, maltase and glucoamylase) which are involved in diabetes. The disadvantages related with the use of synthetic drugs make it necessary to search for alternative drugs from medicinal plants. One therapeutic approach for the treatment of postprandial hyperglycaemia is to decrease the plasma glucose level which can be attained by lowering the starch hydrolysis by digestive enzymes such as α-amylase, maltase and glucoamylase. The use of synthetic antidiabetic agents caused gastrointestinal side effects.  Methods: Hence, the research was aimed to evaluate the potential of Phaseolus lunatus seeds extract to inhibit α-amylase, maltase and glucoamylase as a possible mechanism of hypoglycemia caused by this plant with less side effects.  Phaseolus lunatus, also known as Lima bean, is native to Tropical America and are grown throughout the world. Lima bean has been prescribed traditionally to treat different diseases including diabetes. Result: The results of the present study showed that Phaseolus lunatus seeds extract have exerted both time and concentration dependent significant inhibitory effect on these digestive enzymes which might be due to cumulative effect of phytoconstituents present in the plant extract. Conclusion: Therefore, it can be said that the part of mechanisms by which Phaseolus lunatus exhibited its antidiabetic activity might be through the inhibition of these digestive enzyme.


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How to Cite

Mehreen Zaheer, Anum Kalim, Shah Ali Ul Qadar, Ahmed, S., & Muhammad Mohtasheem Ul Hasan. (2023). Inhibitory potential of Phaseolus lunatus L. Seeds to Digestive Enzymes. BioSight, 4(2-3), 59–69.



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