Inhibitory potential of Phaseolus lunatus L. Seeds to Digestive Enzymes

Authors

  • Mehreen Zaheer Department of Pharmacognosy, Jinnah University for Women, Nazimabad, Karachi-74600, Pakistan
  • Anum Kalim Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, University of Karachi
  • Shah Ali Ul Qadar Department of Biochemistry, University of Karachi
  • Salman Ahmed Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, University of Karachi, Karachi, Pakistan
  • Muhammad Mohtasheem Ul Hasan Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, University of Karachi, Karachi, Pakistan https://orcid.org/0000-0001-5393-7401

DOI:

https://doi.org/10.46568/bios.v4i2-3.87

Keywords:

Phaseolus lunatus, α-amylase, maltase, glucoamylase, diabetes

Abstract

Introduction: Study was taken with an objective to investigate the inhibitory effect of Phaseolus lunatus seeds extract on three enzymes (α-amylase, maltase and glucoamylase) which are involved in diabetes. The disadvantages related with the use of synthetic drugs make it necessary to search for alternative drugs from medicinal plants. One therapeutic approach for the treatment of postprandial hyperglycaemia is to decrease the plasma glucose level which can be attained by lowering the starch hydrolysis by digestive enzymes such as α-amylase, maltase and glucoamylase. The use of synthetic antidiabetic agents caused gastrointestinal side effects.  Methods: Hence, the research was aimed to evaluate the potential of Phaseolus lunatus seeds extract to inhibit α-amylase, maltase and glucoamylase as a possible mechanism of hypoglycemia caused by this plant with less side effects.  Phaseolus lunatus, also known as Lima bean, is native to Tropical America and are grown throughout the world. Lima bean has been prescribed traditionally to treat different diseases including diabetes. Result: The results of the present study showed that Phaseolus lunatus seeds extract have exerted both time and concentration dependent significant inhibitory effect on these digestive enzymes which might be due to cumulative effect of phytoconstituents present in the plant extract. Conclusion: Therefore, it can be said that the part of mechanisms by which Phaseolus lunatus exhibited its antidiabetic activity might be through the inhibition of these digestive enzyme.

References

Krentz, A.J. and C.J. Bailey, Oral Antidiabetic Agents: : current role in type 2 diabetes mellitus. Drugs, 2005. 65(3): p. 385-411.

Gurung, N., et al., A broader view: microbial enzymes and their relevance in industries, medicine, and beyond. BioMed research international, 2013. 2013: p. 1-18.

Rahimzadeh, M., et al., Evaluation of alpha-amylase inhibition by Urtica dioica and Juglans regia extracts. Iranian Journal of Basic Medical Sciences, 2014. 17(6): p. 465-469.

Lévêque, E., et al., Thermophilic archaeal amylolytic enzymes. Enzyme and microbial technology, 2000. 26(1): p. 3-14.

Kumar, P. and T. Satyanarayana, Microbial glucoamylases: characteristics and applications. Critical Reviews in Biotechnology, 2009. 29(3): p. 225-255.

He, Z., et al., An Automated Method for the Determination of Intestinal Disaccharidase and Glucoamylase Activities. Journal of Automated Methods and Management in Chemistry, 2006. 2006: p. 093947.

Agarwal, P. and R. Gupta, Alpha-amylase inhibition can treat diabetes mellitus. Res. Rev. J. Med. Health Sci, 2016. 5(4): p. 1-8.

Poovitha, S. and M. Parani, In vitro and in vivo α-amylase and α-glucosidase inhibiting activities of the protein extracts from two varieties of bitter gourd (Momordica charantia L.). BMC Complementary and Alternative Medicine, 2016. 16(1): p. 185.

Martin, F., et al., Antioxidant and postprandial glucose-lowering potential of the hydroethanolic extract of Nypa fruticans seed mesocarp. Biol Med (Aligarh), 2017. 9: p. 4.

Achan, J., et al., Quinine, an old anti-malarial drug in a modern world: role in the treatment of malaria. Malaria journal, 2011. 10: p. 144-144.

Mangesh, A.B., et al., In vitro studies on alpha amylase inhibitory activity of some indigenous plants. Modern Applications in Pharmacy and Pharmacology, 2018. 1(4): p. 1-5.

S, A., Papillionaceae, in Flora of West Pakistan. Vol. 100, E. Nasir and S. Ali, Editors. 1977: Department of Botany, University of Karachi. p. 239-252.

Saleem, Z.M., S. Ahmed, and M.M. Hasan, Phaseolus lunatus linn: Botany, medicinal uses, phytochemistry and pharmacology. World Journal of Pharmacy and Pharmaceutical Sciences, 2016. 5(11): p. 87-93.

Ezema, B.E., et al., Total Alkaloids, Tannin Content, and Antiulcer Assay of Four Selected Medicinal Plants in Nigeria. Pacific Journal of Science and Technology, 2018. 19(2): p. 284-294.

Seidu, K.T., et al., Chemical composition, phytochemical constituents and antioxidant potentials of lima bean seeds coat. Ann. Food Sci. Technol, 2014. 15(2): p. 288-298.

Trinder, P., Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Annals of Clinical Biochemistry, 1969. 6(1): p. 24-27.

Kazeem, M.I., et al., In-vitro studies on Calotropis procera leaf extracts as inhibitors of key enzymes linked to diabetes mellitus. Iranian journal of pharmaceutical research: IJPR, 2016. 15(Suppl): p. 37-44.

Moein, S., et al., Evaluation of Antioxidant Potentials and α-Amylase Inhibition of Different Fractions of Labiatae Plants Extracts: As a Model of Antidiabetic Compounds Properties. BioMed Research International, 2017. 2017: p. 7319504.

Angelita, D. and M.B. Pricila, Inhibition of digestive enzymes by medicinal plant aqueous extracts used to aid the treatment of obesity. Journal of Medicinal Plants Research, 2012. 6(47): p. 5826-5830.

Oboh, H. and C. Omofoma, The effects of heat treated lima beans (Phaseolus lunatus) on plasma lipids in hypercholesterolemic rats. Pakistan Journal of Nutrition, 2008. 7(5): p. 636-639.

Keerthana, G., M. Kalaivani, and A. Sumathy, In-vitro alpha amylase inhibitory and anti-oxidant activities of ethanolic leaf extract of Croton bonplandianum. Asian Journal of Pharmaceutical and Clinical Research, 2013. 6(4): p. 32-36.

Nair, S.K.P., et al., Preliminary phytochemical screening of different solvent extracts of leaves of Echeveria elegans rose, an endangered mexican succulent herb. J. Glob. Biosci, 2016. 5(1): p. 3429-3432.

Liu, R. and B. Xu, Inhibitory Effects of Phenolics and Saponins From Commonly Consumed Food Legumes in China Against Digestive Enzymes Pancreatic Lipase and α-Glycosidase. International Journal of Food Properties, 2015. 18(10): p. 2246-2255.

Simao, A.A., et al., Aqueous extract of Psidium guajava leaves: phenolic compounds and inhibitory potential on digestive enzymes. Anais da Academia Brasileira de Ciências, 2017. 89(3): p. 2155-2165.

Barrett, A., et al., Inhibition of α-amylase and glucoamylase by tannins extracted from cocoa, pomegranates, cranberries, and grapes. Journal of Agricultural and Food Chemistry, 2013. 61(7): p. 1477-1486.

Kwon, Y.-I., et al., Health benefits of traditional corn, beans, and pumpkin: in vitro studies for hyperglycemia and hypertension management. Journal of Medicinal Food, 2007. 10(2): p. 266-275.

Talukdar, D., Antioxidant potential and type II diabetes related enzyme inhibition properties of raw and processed legumes in Indian Himalayas. Journal of Applied Pharmaceutical Science, 2013. 3(3): p. 13-19.

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Published

2023-07-27

How to Cite

Mehreen Zaheer, Anum Kalim, Shah Ali Ul Qadar, Ahmed, S., & Muhammad Mohtasheem Ul Hasan. (2023). Inhibitory potential of Phaseolus lunatus L. Seeds to Digestive Enzymes. BioSight, 4(2-3), 59–69. https://doi.org/10.46568/bios.v4i2-3.87

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