Heat Inactivation of Avian Influenza (H7N3) Virus In Experimentally Infected Chicken Meat
DOI:
https://doi.org/10.46568/bios.v5i1.163Keywords:
Avian influenza virus, H7N3, Heat inactivationAbstract
Introduction Avian influenza virus (H7N3) cause severe systemic disease in chickens and can be persisted in infected chicken meat that cause public health concern. Influenza viruses are heat liable but parameters for heat inactivation have not been known. Methodology The study investigated the quantitative heat inactivation of H7N3 virus from experimentally infected chicken meat. Twenty (20) sero-negative broiler chickens of 04 weeks age were divided into two group viz A (control) and B artificially infected by giving 0.1ml of 106 embryo infectious dose 50 (EID50) (H7N3) virus through intranasal route. Birds were slaughtered on day 9 post infection and tissues (trachea, kidney, lungs and liver) were collected. Inactivation curves were determined at temperature 60, 61, 62, 63 and 64°C. Results Based on D values, time to inactivate H7N3 virus depends on viral titers and inversely related to temperature. This study investigated confirmed that H7N3 virus was effectively inactivated in chicken meat at 60°C in 4 minutes while at 64°C in 1 minutes. Moreover, protocols per log EID50/g reduction in terms of D values at 60 and 64°C were noted 120 and 30 sec, the coefficient of regression ranged as 0.941 to 0.889.
References
Antarasena C, Sirimujalin R, Prommuang P, Blacksell SD, Promkuntod N, Prommuang P. Tissue tropism of a Thailand strain of high-pathogenicity avian influenza virus (H5N1) in tissues of naturally infected native chickens (Gallus gallus), Japanese quail (Coturnix coturnix japonica) and ducks (Anas spp.). Avian Pathol 2006; 35(3): 250-3.
Tumpey TM, Suarez DL, Perkins LE, Senne DA, Lee JG, Lee YJ, et al. Characterization of a highly pathogenic H5N1 avian influenza A virus isolated from duck meat. J of Virol 2002; 76(12): 6344-55.
Swayne DE, Pantin-Jackwood M. Pathogenicity of avian influenza viruses in poultry. Dev Biol 2006; 124: 61-7.
Swayne DE. Occupational and consumer risks from avian influenza viruses. Dev Biol 2006; 124: 85-90.
Perdue ML, Swayne DE. Public health risk from avian influenza viruses. Avian Dis 2005; 49(3): 317-27.
de Jong MD, Cam BV, Qui PT, Hien VM, Thanh TT, Hue NB, et al. Fatal avian influenza A (H5N1) in a child presenting with diarrhea followed by coma. N Engl J Med 2005; 352(7): 686-91.
Bertran K, Swayne DE. High doses of highly pathogenic avian influenza virus in chicken meat are required to infect ferrets. Vet Res 2014; 45(1): 1-4.
Hayden F, Croisier A. Transmission of avian influenza viruses to and between humans. Infect Dis 2005; 192(8): 1311-4.
OIE, 2005. Office International des Epizooties. 2005. Terrestrial Animal Health Code, 2005. Available at: http://www.oie.int/eng/normes/mcode/ensommaire.htm. Accessed 21 June 2006.
Swayne DE, Beck JR. Experimental study to determine if low-pathogenicity and high-pathogenicity avian influenza viruses can be present in chicken breast and thigh meat following intranasal virus inoculation. Avian Dis 2005; 49(1): 81-5.
Balish AL, Katz JM, Klimov AI. Influenza: propagation, quantification, and storage. Curr Protoc Microbiol 2013; 29(1): 15G-.
Swayne DE, Halvorson DA. Influenza In “Disease of Poultry” Ed. By SM Saif, HJ Barnes, AM Fadly, JR Glisson, LR McDougald, DE Swayne, 2003; 135-60.
Reed LJ, Muench H. A simple method of estimating fifty per cent endpoints. Am J Epidemiol 1938; 27(3): 493-7.
Rimmelzwaan GF, van Riel D, Baars M, Bestebroer TM, van Amerongen G, Fouchier RA, et al. Influenza A virus (H5N1) infection in cats causes systemic disease with potential novel routes of virus spread within and between hosts. Am J of Pathol 2006; 168(1): 176-83.
Songserm T, Amonsin A, Jam-on R, Sae-Heng N, Meemak N, Pariyothorn N, et al. Avian influenza H5N1 in naturally infected domestic cat. J Emerg Infect Dis 2006; 12(4): 681.
Keawcharoen J, Oraveerakul K, Kuiken T, Fouchier RA, Amonsin A, Payungporn S, et al. Avian influenza H5N1 in tigers and leopards. J Emerg Infect Dis 2004; 10(12): 2189.
Thomas C, Swayne DE. Thermal inactivation of H5N2 high-pathogenicity avian influenza virus in dried egg white with 7.5% moisture. J Food Prot 2009; 72(9): 1997-2000.
Chmielewski RA, Beck JR, Swayne DE. Thermal inactivation of avian influenza virus and Newcastle disease virus in a fat-free egg product. J Food Prot 2011; 74(7): 1161-9.
Thomas C, King DJ, Swayne DE. Thermal inactivation of avian influenza and Newcastle disease viruses in chicken meat. J Food Prot 2008; 71(6): 1214-22.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Amjad Ali Channa, Atta Hussain Shah, Ayaz Hussain Mangi, Nazeer Hussain Kalhoro, Zaheer Ahmed Nizamani, Hidayatullah Soomro, Muneer Ahmed Jamali
This work is licensed under a Creative Commons Attribution 4.0 International License.