Assessment of Silent Information Regulator of Transcription Polymorphism Frequency Among Female Hypertensive Patients: A Cross-Sectional Study
DOI:
https://doi.org/10.46568/bios.v6i1-2.227Keywords:
Hypertension, SIRT, Obesity, PregnancyAbstract
The prevalence of Silent Information Regulator of Transcription Polymorphism (SIRT) variants about obesity and hypertension susceptibility remains a subject of interest. This study evaluated the frequency of the SIRT1 rs369274325 polymorphism among hypertensive pregnant females. In this cross-sectional study at Aga Khan University from March 2017 to May 2020, 120 pregnant women aged 18-45 were grouped into Normal Weight Hypertensive, Obese Hypertensive, Normal Weight Normotensive, and Obese Normotensive categories. Excluding individuals with pre-existing diabetes, gestational diabetes, inflammatory conditions, or using oral contraception/hormonal support, venous blood (3ml) was collected for DNA extraction. Tetra arms polymerase chain reaction (T-arms PCR) targeted SIRT1 rs369274325 polymorphism, with subsequent agarose gel electrophoresis for PCR product analysis. While age matching was achieved across groups (p= >0.05), differences were seen for BMI, systolic, and diastolic blood pressure among the groups (p= <0.05). No disparities in allelic and genotypic distributions of the SIRT1 polymorphism were detected across the four groups, with a minor allele frequency (MAF) of 0 (p=1.00). The findings suggest that the SIRT1 rs369274325 GG genotype prevails within the local population and may not exhibit a direct association with hypertension.
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Copyright (c) 2025 Ibrahim Munaf Ahmed , Muhammad Saad Hafeez , Syeda Sadia Fatima
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